Lysine Ornithine Mannitol Agar (LOM Agar)

[所属分类:培养基配方] [发布时间:2021-5-10] [发布人:网站管理员2] [阅读次数:] [返回]

Lysine Ornithine Mannitol Agar
(LOM Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................13.5g
L –Ornithine·HCl ...........................................................................6.5g
D –Mannitol.................................................................................5.25g
L –Lysine·HCl................................................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................3.0g
Bromthymol Blue.........................................................................0.3g
Vancomycin solution ...............................................................10.0mL
pH 6.5 ± 0.2 at 25°C
Vancomycin Solution:
Composition per 10.0mL:
Vancomycin·HCl.........................................................................0.03g
Preparation of Vancomycin Solution: Add vancomycin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except vancomycin so-
lution, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile van-
comycin solution. Mix thoroughly. Pour into sterile Petri dishes or dis-
tribute into sterile tubes.
Use: For the cultivation and differentiation of Gram-negative bacilli
based on their ability to decarboxylate lysine or ornithine and mannitol
fermentation. Especially useful for the identification of Enterobacter
agglomerans. Bacteria that ferment mannitol appear as dark yellow
colonies. Bacteria that decarboxylate lysine or ornithine appear as
green-yellow colonies.