Lysine Medium

[所属分类:培养基配方] [发布时间:2021-5-10] [发布人:网站管理员2] [阅读次数:] [返回]

Lysine Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .......................................................................................44.5g
Agar ............................................................................................17.8g
KH 2 PO 4 .......................................................................................1.78g
Lysine............................................................................................1.0g
MgSO 4 ·7H 2 O..............................................................................0.89g
CaCl 2 ·2H 2 O...............................................................................0.178g
NaCl..........................................................................................0.089g
Inositol........................................................................................0.02g
Calcium pantothenate ................................................................2.0mg
Adenine....................................................................................1.78mg
DL- Methionine..........................................................................0.89mg
L- Histidine................................................................................0.89mg
DL- Tryptophan..........................................................................0.89mg
Thiamine·HCl ............................................................................0.4mg
Pyridoxine..................................................................................0.4mg
Nicotinic acid.............................................................................0.4mg
FeSO 4 ·7H 2 O.............................................................................0.22mg
p-Aminobenzoic acid.................................................................0.2mg
Riboflavin ..................................................................................0.2mg
MnSO 4 ·H 2 O...........................................................................0.035mg
ZnSO 4 ·7H 2 O..........................................................................0.035mg
(NH 4 ) 2 MoO 4 ·4H 2 O................................................................0.018mg
H 3 BO 3 .........................................................................................8.9μg
Biotin ..........................................................................................2.0μg
Folic acid.....................................................................................1.0μg
Potassium lactate (50% solution).............................................10.0mL
Lactic acid..................................................................................1.0mL
pH 4.8 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium: Add potassium lactate to distilled/deion-
ized water and bring volume to 900.0mL. Mix thoroughly. Add re-
maining components, except lactic acid. Mix thoroughly. Gently heat
while stirring and bring to boiling. Do not autoclave. Cool to 50°C. Ad-
just pH to 4.8 by adding 1.0mL of lactic acid. Pour into sterile Petri
dishes. Dry the surface of the plates by incubation at 37°C for 24 hr.
Use: For the isolation, cultivation, and enumeration of wild yeasts
encountered in brewing.