Lysine Decarboxylase Medium

[所属分类:培养基配方] [发布时间:2021-5-10] [发布人:网站管理员2] [阅读次数:] [返回]

Lysine Decarboxylase Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .........................................................................................0.5g
KH 2 PO 4 .........................................................................................0.5g
L- Lysine·HCl.................................................................................0.5g
pH 4.6 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Adjust pH to 4.6. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically distribute into sterile tubes in 1.0mL volumes.
Use: For the cultivation and differentiation of Gram-negative, nonfer-
mentative bacteria based on their ability to decarboxylate lysine. Bac-
teria that decarboxylate lysine turn the medium turbid purple.