Lysine Iron Agar

[所属分类:培养基配方] [发布时间:2021-5-10] [发布人:网站管理员2] [阅读次数:] [返回]

Lysine Iron Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................13.5g
L- Lysine.......................................................................................10.0g
Pancreatic digest of gelatin...........................................................5.0g
Yeast extract..................................................................................3.0g
Glucose .........................................................................................1.0g
Ferric ammonium citrate...............................................................0.5g
Na 2 S 2 O 3 ·5H 2 O............................................................................0.04g
Bromcresol Purple ......................................................................0.02g
pH 6.7 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems and Oxoid Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Distribute into tubes in 10.0mL volumes.
Autoclave for 12 min at 15 psi pressure–121°C. Allow tubes to cool in

a slanted position.

Use: For the cultivation and differentiation of members of the Enter-
obacteriaceae based on their ability to decarboxylate lysine and to form
H 2 S. Bacteria that decarboxylate lysine turn the medium purple. Bac-
teria that produce H 2 S appear as black colonies.