Lysine Decarboxylase HiVeg Broth

[所属分类:培养基配方] [发布时间:2021-5-10] [发布人:网站管理员2] [阅读次数:] [返回]

Lysine Decarboxylase HiVeg Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Plant peptone.................................................................................5.0g
L -Lysine hydrochloride.................................................................5.0g
Yeast extract..................................................................................3.0g
Glucose .........................................................................................1.0g
Bromcresol Purple ......................................................................0.02g
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMe-
dia.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically
distribute into sterile tubes in 1.0mL volumes.
Use: For the cultivation and differentiation of Gram-negative, nonfer-
mentative bacteria based on their ability to decarboxylate lysine. Bac-
teria that decarboxylate lysine turn the medium purple.