Lysine Decarboxylase Broth, Falkow

[所属分类:培养基配方] [发布时间:2021-5-10] [发布人:网站管理员2] [阅读次数:] [返回]

Lysine Decarboxylase Broth, Falkow

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone .........................................................................................5.0g
L- Lysine.........................................................................................5.0g
Yeast extract..................................................................................3.0g
Glucose.........................................................................................1.0g
Bromcresol Purple......................................................................0.02g
pH 6.5–6.8 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Adjust pH to 6.5–6.8. Distribute into tubes in 5.0mL vol-
umes. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and differentiation of bacteria, especially Sal-
monella, based on their ability to decarboxylate lysine. Bacteria that
decarboxylate lysine turn the medium turbid purple.