Lysine Decarboxylase Broth, Taylor Modification

[所属分类:培养基配方] [发布时间:2021-5-10] [发布人:网站管理员2] [阅读次数:] [返回]

Lysine Decarboxylase Broth, Taylor Modification

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

L- Lysine.........................................................................................5.0g
Yeast extract..................................................................................3.0g
Glucose.........................................................................................1.0g
Bromcresol Purple......................................................................0.02g
pH 6.1 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Adjust pH to 6.1. Distribute into tubes in 5.0mL volumes.
Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and differentiation of bacteria, especially Sal-
monella, based on their ability to decarboxylate lysine. Bacteria that
decarboxylate lysine turn the medium turbid purple.