Lysine Broth Falkow with Sodium Chloride (BAM M44)

[所属分类:培养基配方] [发布时间:2021-5-10] [发布人:网站管理员2] [阅读次数:] [返回]

Lysine Broth Falkow with Sodium Chloride
(BAM M44)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

L -Lysine.........................................................................................5.0g
Peptone or gelysate.......................................................................5.0g
Yeast extract..................................................................................3.0g
Glucose .........................................................................................1.0g
Bromcresol Purple ......................................................................0.02g

pH 6.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Adjust pH so that is will be
6.5 ± 0.2 after sterilization. Distribute into 16 × 150mm screw-capped
tubes in 5.0mL volumes. Autoclave medium with loosely capped tubes for
10 min at 15 psi pressure–121°C. Screw the caps on tightly for storage and
after inoculation.
Use : For the cultivation and differentiation of Vibrio spp. based on
their ability to decarboxylate the amino acid lysine. Bacteria that decar-
boxylate lysine turn the medium turbid purple.