Lysine Arginine Iron HiVeg Agar

[所属分类:培养基配方] [发布时间:2021-5-10] [发布人:网站管理员2] [阅读次数:] [返回]

Lysine Arginine Iron HiVeg Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
L -Arginine...................................................................................10.0g
L -Lysine.......................................................................................10.0g
Plant peptone.................................................................................5.0g
Yeast extract..................................................................................3.0g
Glucose .........................................................................................1.0g
Ferric ammonium citrate...............................................................0.5g
Na 2 S 2 O 3 ......................................................................................0.04g
Bromcresol Purple ......................................................................0.02g
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMe-
dia.
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to
boiling. Adjust pH to 6.8. Distribute into screw-capped tubes in 5.0mL
volumes. Autoclave for 12 min at 15 psi pressure–121°C. Allow tubes to
cool in a slanted position.
Use: For the cultivation and differentiation of bacteria based on their abil-
ity to decarboxylate lysine, decarboxylate arginine, and produce H 2 S. Bac-
teria that decarboxylate lysine or arginine turn the medium purple.