Lombard-Dowell Gelatin Agar (LD Gelatin Agar)

[所属分类:培养基配方] [发布时间:2021-5-8] [发布人:网站管理员2] [阅读次数:] [返回]

Lombard-Dowell Gelatin Agar
(LD Gelatin Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................20.0g
Pancreatic digest of casein............................................................5.0g
Yeast extract..................................................................................5.0g
Gelatin...........................................................................................4.0g
NaCl..............................................................................................2.5g
Glucose.........................................................................................1.0g
L- Cystine.......................................................................................0.4g
L- Tryptophan.................................................................................0.2g
Na 2 SO 3 ..........................................................................................0.1g
Hemin ......................................................................................10.0mg
NaOH (1N).................................................................................5.0mL
Vitamin K 1  solution....................................................................1.0mL

pH 7.5 ± 0.2 at 25°C

Vitamin K 1 Solution:
Composition per 100.0mL:
Vitamin K 1 ....................................................................................1.0g
Ethanol.....................................................................................99.0mL
Preparation of Vitamin K 1 Solution:  Add vitamin K 1 to 99.0mL
of absolute ethanol. Mix thoroughly.
Preparation of Medium: Add hemin and  L -cystine to 5.0mL of
NaOH. Mix thoroughly. Add remaining components, except agar and
gelatin. Bring volume to 750.0mL with distilled/deionized water. Mix
thoroughly. Gently heat and bring to boiling. In a separate flask, add gel-
atin to 100.0mL of cold distilled/deionized water. Gently heat and bring
to 70°C. Add gelatin solution to the 750.0mL of basal medium. Mix thor-
oughly. Add agar. Bring volume to 1.0L with distilled/deionized water.
Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri
dishes.
Use: For the cultivation of a wide variety of anaerobic bacteria. For the
differentiation of anaerobic bacteria based on gelatinase production.
After incubation of plates, gelatinase activity is determined by the
addition of Frazier’s reagent. Bacteria that hydrolyze gelatin appear as
colonies surrounded by a clear zone.