Lombard-Dowell Neomycin Agar (Egg Yolk Agar with Neomycin)

[所属分类:培养基配方] [发布时间:2021-5-8] [发布人:网站管理员2] [阅读次数:] [返回]

Lombard-Dowell Neomycin Agar
(Egg Yolk Agar with Neomycin)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 9100.0mL:

Na 2 HPO 4 ·12H 2 O...........................................................................5.0g
Glucose .........................................................................................2.0g
Neomycin sulfate..........................................................................0.1g
LD Agar...............................................................................9000.0mL
Egg yolk emulsion.................................................................100.0mL
MgSO 4 ·7H 2 0 (5% solution).......................................................0.2mL
pH 7.5 ± 0.2 at 25°C
LD Agar:
Composition per liter:
Agar ............................................................................................20.0g
Pancreatic digest of casein............................................................5.0g
Yeast extract..................................................................................5.0g
NaCl..............................................................................................2.5g
L- Cystine .......................................................................................0.4g
L- Tryptophan.................................................................................0.2g
Na 2 SO 3 ..........................................................................................0.1g
Hemin.......................................................................................10.0mg
NaOH (1N NaOH).....................................................................5.0mL
Vitamin K 1  solution....................................................................1.0mL
Preparation of LD Agar: Add hemin and  L -cystine to 5.0mL of
NaOH. Mix thoroughly. Add remaining components. Mix thoroughly.
Gently heat and bring to boiling.
Vitamin K 1 Solution:
Composition per 100.0mL:
Vitamin K 1 ....................................................................................1.0g
Ethanol.....................................................................................99.0mL
Preparation of Vitamin K 1 Solution:  Add vitamin K 1 to 99.0mL
of absolute ethanol. Mix thoroughly.
Egg Yolk Emulsion:
Composition :
Chicken egg yolks............................................................................11
Whole chicken egg.............................................................................1
Preparation of Egg Yolk Emulsion:  Soak eggs with 1:100 dilu-
tion of saturated mercuric chloride solution for 1 min. Crack eggs and
separate yolks from whites. Mix egg yolks with 1 chicken egg.
Preparation of Medium: Combine components, except egg yolk
emulsion and neomycin sulfate. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL
of egg yolk emulsion and neomycin sulfate. Mix thoroughly. Pour into
sterile Petri dishes.
Use: For the selective cultivation of a wide variety of anaerobic bacte-
ria. For the differentiation of anaerobic bacteria based on lecithinase
production, lipase production, and proteolytic ability. Bacteria that
produce lecithinase appear as colonies surrounded by a zone of insolu-
ble precipitate. Bacteria that produce lipase appear as colonies with a
pearly iridescent sheen. Bacteria that produce proteolytic activity
appear as colonies surrounded by a clear zone.