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培养基配方
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Lombard-Dowell Esculin Agar (LD Esculin Agar) (Esculin Agar, Lombard-Dowell)
[所属分类:培养基配方] [发布时间:2021-5-8] [发布人:网站管理员2] [阅读次数:] [返回]
Lombard-Dowell Esculin Agar
(LD Esculin Agar)
(Esculin Agar, Lombard-Dowell)
Pancreatic digest of casein............................................................5.0g
Yeast extract..................................................................................5.0g
NaCl..............................................................................................2.5g
Esculin ..........................................................................................1.0g
Ferric citrate..................................................................................0.5g
L- Cystine.......................................................................................0.4g
L- Tryptophan.................................................................................0.2g
Hemin ......................................................................................10.0mg
NaOH (1N).................................................................................5.0mL
Vitamin K 1 solution....................................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Vitamin K 1 Solution:
Composition per 100.0mL:
Vitamin K 1 ....................................................................................1.0g
Ethanol.....................................................................................99.0mL
Preparation of Vitamin K 1 Solution: Add vitamin K 1 to 99.0mL
of absolute ethanol. Mix thoroughly.
Preparation of Medium: Add hemin and L -cystine to 5.0mL of
NaOH. Mix thoroughly. Add remaining components. Bring volume to
1.0L with distilled/deionized water. Mix thoroughly. Gently heat and
bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dishes.
Use: For the cultivation of a wide variety of anaerobic bacteria. For the
differentiation of anaerobic bacteria based on esculin hydrolysis, H 2 S
production, and catalase production. Bacteria that hydrolyze esculin
appear as colonies surrounded by a red-brown to dark brown zone.
Bacteria that produce H 2 S appear as black colonies.
(LD Esculin Agar)
(Esculin Agar, Lombard-Dowell)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar............................................................................................20.0gPancreatic digest of casein............................................................5.0g
Yeast extract..................................................................................5.0g
NaCl..............................................................................................2.5g
Esculin ..........................................................................................1.0g
Ferric citrate..................................................................................0.5g
L- Cystine.......................................................................................0.4g
L- Tryptophan.................................................................................0.2g
Hemin ......................................................................................10.0mg
NaOH (1N).................................................................................5.0mL
Vitamin K 1 solution....................................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Vitamin K 1 Solution:
Composition per 100.0mL:
Vitamin K 1 ....................................................................................1.0g
Ethanol.....................................................................................99.0mL
Preparation of Vitamin K 1 Solution: Add vitamin K 1 to 99.0mL
of absolute ethanol. Mix thoroughly.
Preparation of Medium: Add hemin and L -cystine to 5.0mL of
NaOH. Mix thoroughly. Add remaining components. Bring volume to
1.0L with distilled/deionized water. Mix thoroughly. Gently heat and
bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dishes.
Use: For the cultivation of a wide variety of anaerobic bacteria. For the
differentiation of anaerobic bacteria based on esculin hydrolysis, H 2 S
production, and catalase production. Bacteria that hydrolyze esculin
appear as colonies surrounded by a red-brown to dark brown zone.
Bacteria that produce H 2 S appear as black colonies.
