Liver Broth

[所属分类:培养基配方] [发布时间:2021-5-7] [发布人:网站管理员2] [阅读次数:] [返回]

Liver Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Beef liver, fresh.........................................................................453.0g
Pancreatic digest of casein..........................................................10.0g
K 2 HPO 4 .........................................................................................1.0g
Soluble starch................................................................................1.0g
pH 7.6 ± 0.2 at 25°C
Preparation of Medium:  Remove the fat from fresh beef liver. Grind
the liver. Add 1.0L of distilled/deionized water. Gently heat and bring to
boiling. Continue boiling for 60 min. Adjust pH to 7.6. Filter through
cheesecloth. Reserve meat. To filtrate, add pancreatic digest of casein,
K 2 HPO 4 , and soluble starch. Bring volume to 1.0L with distilled/deion-
ized water. Refilter solution. Add meat particles to test tubes to a depth
of approximately 2cm. Distribute broth into tubes with meat particles in
15.0mL volumes. Autoclave for 20 min at 15 psi pressure–121°C.
Use: For the isolation and cultivation of anaerobic microorganisms,
especially Clostridium botulinum, from foods.