Liver Broth

[所属分类:培养基配方] [发布时间:2021-5-7] [发布人:网站管理员2] [阅读次数:] [返回]

Liver Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Extracted liver tissue, minced.....................................................30.0g
Infusion from fresh liver.............................................................23.0g
Peptone .......................................................................................10.0g
K 2 HPO 4 .........................................................................................1.0g
Agar overlay solution ............................................................200.0mL
pH 6.8 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Agar Overlay Solution:
Composition per 200.0mL:
Agar, Oxoid Unipath No. 3...........................................................4.0g
Preparation of Agar Overlay Solution: Add agar to distilled/de-
ionized water and bring volume to 200.0mL. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C.
Preparation of Medium: Add components, except agar, to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gen-
tly heat and bring to boiling. Distribute into tubes to a depth of 50mm.
Make sure that some liver particles are transferred to each tube. Auto-
clave for 20 min at 10 psi pressure–115°C. After inoculation, aseptical-
ly overlay the broth with 2.0mL of sterile, cooled agar solution per
tube.
Use: For the isolation and cultivation of saccharolytic or putrefactive
mesophilic and thermophilic anaerobic bacteria from foods.