Liver Broth with Sodium Chloride

[所属分类:培养基配方] [发布时间:2021-5-7] [发布人:网站管理员2] [阅读次数:] [返回]

Liver Broth with Sodium Chloride

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Beef liver, fresh.........................................................................453.0g
NaCl..........................................................................................150.0g
Pancreatic digest of casein..........................................................10.0g
K 2 HPO 4 .........................................................................................1.0g
Soluble starch................................................................................1.0g
pH 7.6 ± 0.2 at 25°C
Preparation of Medium:  Remove the fat from fresh beef liver.
Grind the liver. Add 1.0L of distilled/deionized water. Gently heat and
bring to boiling. Continue boiling for 60 min. Adjust pH to 7.6. Filter
through cheesecloth. Reserve meat. To filtrate, add pancreatic digest of
casein, K 2 HPO 4 , NaCl, and soluble starch. Bring volume to 1.0L with
distilled/deionized water. Refilter solution. Add meat particles to test
tubes to a depth of approximately 2cm. Distribute broth into tubes with
meat particles in 15.0mL volumes. Autoclave for 20 min at 15 psi pres-
sure–121°C. After inoculation, overlay each tube with sterile, melted
petroleum jelly.
Use: For the cultivation of obligate halophiles from brined and dry-
salted vegetables.