Listeria Selective HiVeg Broth Base with Antibiotic Inhibitor

[所属分类:培养基配方] [发布时间:2021-5-7] [发布人:网站管理员2] [阅读次数:] [返回]

Listeria Selective HiVeg Broth Base
with Antibiotic Inhibitor

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Plant hydrolysate ........................................................................17.0g
Yeast extract..................................................................................6.0g
NaCl..............................................................................................5.0g
Papaic digest of soybean meal......................................................3.0g
Glucose.........................................................................................2.5g
K 2 HPO 4 .........................................................................................2.5g
Antibiotic inhibitor ..................................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium, without antibiotic inhibitor, is available as a
premixed powder from HiMedia.
Antibiotic Inhibitor:
Composition per 10.0mL:
Cycloheximide..............................................................................0.4g
Colistin sulfate............................................................................0.02g
Fosfomycin.................................................................................0.01g
Acriflavine.................................................................................5.0mg
Cefotetan....................................................................................2.0mg
Ethanol (50% solution)............................................................10.0mL
Preparation of Antibiotic Inhibitor: Add antibiotics to 10.0mL
of ethanol. Mix thoroughly. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except antibiotic inhib-
itor, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of
sterile antibiotic inhibitor. Mix thoroughly. Distribute into sterile tubes.
Use: For the cultivation of Listeria monocytogenes from specimens
containing a mixed bacterial flora. For the cultivation of Listeria spe-
cies from pathological specimens.