Listeria Transport Enrichment Medium

[所属分类:培养基配方] [发布时间:2021-5-7] [发布人:网站管理员2] [阅读次数:] [返回]

Listeria Transport Enrichment Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sodium glycerophosphate...........................................................10.0g
Agar..............................................................................................2.0g
Sodium thioglycolate....................................................................1.0g
CaCl 2 .............................................................................................0.1g
Nalidixic acid..............................................................................0.04g
Acridine solution .......................................................................2.0mL
pH 7.4 ± 0.2 at 25°C
Acridine Solution:
Composition per 10.0mL:
Acridine......................................................................................0.04g
Preparation of Acridine Solution: Add acridine to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium: Add components, except acridine solu-
tion, to distilled/deionized water and bring volume to 998.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 2.0mL acri-
dine solution. Mix thoroughly. Aseptically distribute into sterile tubes
in 10.0mL volumes or fill bottles 4/5 full.
Use: For the maintenance—as a transport medium—and enrichment
of Listeria species.