Listeria Selective HiVeg Agar

[所属分类:培养基配方] [发布时间:2021-5-7] [发布人:网站管理员2] [阅读次数:] [返回]

Listeria Selective HiVeg Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Potassium thiocyanate.................................................................37.5g
Agar ............................................................................................13.0g
Plant hydrolysate.........................................................................10.0g
Plant peptone...............................................................................10.0g
NaCl..............................................................................................5.0g
Glucose .........................................................................................1.0g
Nalidixic acid..............................................................................0.04g
Acriflavin hydrochloride (Trypaflavin)......................................0.01g
Thiaminium dichloride ..............................................................5.0mg

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMe-
dia.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the selective isolation and cultivation of Listeria species from
clinical specimens.