Listeria Oxford Medium Base with Antibiotic Inhibitor

[所属分类:培养基配方] [发布时间:2021-5-7] [发布人:网站管理员2] [阅读次数:] [返回]

Listeria Oxford Medium Base
with Antibiotic Inhibitor

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone, special ..........................................................................23.0g
LiCl.............................................................................................15.0g
Agar ............................................................................................10.0g
NaCl..............................................................................................5.0g
Cornstarch.....................................................................................1.0g
Esculin ..........................................................................................1.0g
Ammonium ferric citrate ..............................................................0.5g
Antibiotic inhibitor ..................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium, without antibiotic inhibitor, is available as a
premixed powder from HiMedia.
Caution:  LiCl is harmful. Avoid bodily contact and inhalation of va-
pors. On contact with skin wash with plenty of water immediately.
Antibiotic Inhibitor:
Composition per 10.0mL:
Cycloheximide..............................................................................0.4g
Colistin sulfate............................................................................0.02g
Fosfomycin .................................................................................0.01g
Acriflavine.................................................................................5.0mg
Cefotetan....................................................................................2.0mg
Ethanol (50% solution)............................................................10.0mL
Preparation of Antibiotic Inhibitor: Add antibiotics to 10.0mL
of ethanol. Mix thoroughly. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except antibiotic inhib-
itor, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of
sterile antibiotic inhibitor. Mix thoroughly. Pour into sterile Petri dish-
es or distribute into sterile tubes.
Use: For the isolation and cultivation of Listeria monocytogenes from
specimens containing a mixed bacterial flora. For the isolation of List-
eria species from pathological specimens.