Levinthal’s Agar

[所属分类:培养基配方] [发布时间:2021-5-6] [发布人:网站管理员2] [阅读次数:] [返回]

Levinthal’s Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 105.0mL:

Nutrient agar, sterile ..............................................................100.0mL
Rabbit blood or human blood, sterile ........................................5.0mL
pH 6.8 ± 0.2 at 25°C
Nutrient Agar:
Composition per liter:
Agar............................................................................................15.0g
Pancreatic digest of gelatin...........................................................5.0g
Beef extract...................................................................................3.0g
Source:  Nutrient agar is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Nutrient Agar: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Gently heat
while stirring and bring to boiling. Distribute into tubes or flasks. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium: To 100.0mL of cooled, sterile nutrient
agar, aseptically add 5.0mL of human blood or rabbit blood. Mix thor-
oughly. Heat in a boiling water bath for 5 min. Allow the deposit to set-
tle out of suspension. Pour the clear supernatant solution into sterile
Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Haemophilus species.