- 定做培养基/定制培养基
- 颗粒培养基
- 标准菌株生化鉴定试剂盒
- 预灌装即用型成品培养基
- 2025年版中国药典
- 促销/特价商品
- 院感/疾控/体外诊断/采样管
- 样品采集与处理(均质)产品
- 按标准检索培养基
- 模拟灌装用培养基
- 干燥粉末培养基
- 培养基添加剂/补充剂
- 生化反应鉴定管
- 染色液等配套产品
- 对照培养基/标准品
- 实验耗材与器具
- 生化试剂/化学试剂
- 菌种鉴定服务
培养基配方
您现在的位置： 网站首页 >> 培养基配方
Levine EMB Agar (Levine Eosin Methylene Blue Agar) (Eosin Methylene Blue Agar, Levine) (LEMB Agar)
[所属分类:培养基配方] [发布时间:2021-5-6] [发布人:网站管理员2] [阅读次数:] [返回]
Levine EMB Agar
(Levine Eosin Methylene Blue Agar)
(Eosin Methylene Blue Agar, Levine)
(LEMB Agar)
Lactose........................................................................................10.0g
Peptone........................................................................................10.0g
K 2 HPO 4 .........................................................................................2.0g
Eosin Y .........................................................................................0.4g
Methylene Blue......................................................................0.065mg
pH 7.1 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the isolation, cultivation, and differentiation of Gram-negative
enteric bacteria based on lactose fermentation. Bacteria that ferment lac-
tose, especially the coliform bacterium Escherichia coli, appear as colo-
nies with a green metallic sheen or blue-black to brown color. Bacteria
that do not ferment lactose appear as colorless or transparent light purple
colonies.
(Levine Eosin Methylene Blue Agar)
(Eosin Methylene Blue Agar, Levine)
(LEMB Agar)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar ............................................................................................15.0gLactose........................................................................................10.0g
Peptone........................................................................................10.0g
K 2 HPO 4 .........................................................................................2.0g
Eosin Y .........................................................................................0.4g
Methylene Blue......................................................................0.065mg
pH 7.1 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the isolation, cultivation, and differentiation of Gram-negative
enteric bacteria based on lactose fermentation. Bacteria that ferment lac-
tose, especially the coliform bacterium Escherichia coli, appear as colo-
nies with a green metallic sheen or blue-black to brown color. Bacteria
that do not ferment lactose appear as colorless or transparent light purple
colonies.
