Levinthal’s HiVeg Medium Base with Blood

[所属分类:培养基配方] [发布时间:2021-5-6] [发布人:网站管理员2] [阅读次数:] [返回]

Levinthal’s HiVeg Medium Base with Blood

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................20.0g
Plant extract................................................................................10.0g
Plant peptone ..............................................................................10.0g
NaCl..............................................................................................5.0g
Rabbit blood or human blood, sterile ......................................50.0mL
pH 7.6 ± 0.2 at 25°C
Source: This medium, without blood, is available as a premixed pow-
der from HiMedia.
Preparation of Medium: Add components, except blood, to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gen-
tly heat while stirring and bring to boiling. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 50.0mL of human blood or rabbit blood. Mix
thoroughly. Heat in a boiling water bath for 5 min. Allow the deposit
to settle out of suspension. Pour the clear supernatant solution into ster-
ile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Haemophilus species.