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培养基配方
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Leptospira Medium, EMJH (Leptospira Medium, Ellinghausen-McCullough/ Johnson-Harris)
[所属分类:培养基配方] [发布时间:2021-4-30] [发布人:李佳颐] [阅读次数:] [返回]
Leptospira Medium, EMJH
(Leptospira Medium, Ellinghausen-McCullough/
Johnson-Harris)
NaCl..............................................................................................1.0g
KH 2 PO 4 .........................................................................................0.3g
NH 4 Cl .........................................................................................0.25g
Thiamine....................................................................................5.0mg
Rabbit serum..........................................................................100.0mL
pH 7.5 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components, except rabbit serum, to
distilled/deionized water and bring volume to 900.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 25°C. Aseptically add sterile rabbit serum.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Leptospira species.
(Leptospira Medium, Ellinghausen-McCullough/
Johnson-Harris)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Na 2 HPO 4 .......................................................................................1.0gNaCl..............................................................................................1.0g
KH 2 PO 4 .........................................................................................0.3g
NH 4 Cl .........................................................................................0.25g
Thiamine....................................................................................5.0mg
Rabbit serum..........................................................................100.0mL
pH 7.5 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components, except rabbit serum, to
distilled/deionized water and bring volume to 900.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 25°C. Aseptically add sterile rabbit serum.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Leptospira species.
