Leptospira Medium, Modified

[所属分类:培养基配方] [发布时间:2021-4-30] [发布人:李佳颐] [阅读次数:] [返回]

Leptospira Medium, Modified

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..............................................................................................1.5g
NaCl..............................................................................................0.5g
Peptone..........................................................................................0.3g
Beef extract...................................................................................0.2g
Hemin solution...........................................................................2.5mL
Sterile rabbit serum................................................................100.0mL
pH 7.3 ± 0.1 at 25°C
Hemin Solution:
Composition per 100.0mL:
Hemin..........................................................................................0.05g
NaOH (1N solution)...................................................................1.0mL
Preparation of Hemin Solution:  Add hemin to 1.0mL of 1N
NaOH solution. Mix thoroughly. Bring volume to 100.0mL with dis-
tilled/deionized water. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 45°–50°C.
Preparation of Medium: Add components, except hemin solution
and rabbit serum, to distilled/deionized water and bring volume to
897.5mL. Mix thoroughly. Gently heat and bring to boiling. Adjust pH
to 7.4. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 2.5mL of sterile hemin solution and 100.0mL of
sterile rabbit serum. Mix thoroughly. The pH of the medium should be
7.3. Store at 4°C for 24 hr. Inactivate medium at 56°C for 60 min.
Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Leptospira biflexa, Lep-
tospira borgpetersenii, Leptospira interrogans, Leptospira meyeri, Lep-
tospira noguchii, Leptospira santarosai, and Leptospira weili.