Leptospira Medium (DSMZ Medium 1113)

[所属分类:培养基配方] [发布时间:2021-4-30] [发布人:李佳颐] [阅读次数:] [返回]

Leptospira Medium
(DSMZ Medium 1113)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agarose.........................................................................................1.5g
Na 2 HPO 4 .......................................................................................1.0g
NaCl..............................................................................................1.0g
KH 2 PO 4 .........................................................................................0.3g
NH 4 Cl .........................................................................................0.25g
Thiamine....................................................................................5.0mg
Leptospira enrichment EMJH (a solution of albumin,
polysorbate 80 and additional growth factors
available from BD Diagnostics .......................................100.0mL
pH 7.5 ± 0.2 at 25°C
Preparation of Medium: Add agarose to distilled/deionized water
and bring volume to 900.0mL. Mix thoroughly. Gently heat while stir-
ring and bring to boiling. Boil with mixing until agarose dissolves. Add
the other components, except Leptospira enrichment EMJH. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Mix thoroughly. Warm the Leptospira enrichment to approximately
25°C and add to the warm medium. Mix thoroughly. Aseptically dis-
tribute into sterile tubes or flasks.
Use: For the cultivation of Leptospira spp.