Leptospira Medium

[所属分类:培养基配方] [发布时间:2021-4-30] [发布人:李佳颐] [阅读次数:] [返回]

Leptospira Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

(NH 4 ) 2 Fe(SO 4 ) 2 ·6H 2 O..................................................................6.0g
NaH 2 PO 4 .....................................................................................0.53g
L- Asparagine.................................................................................0.5g
Glycerol ........................................................................................0.2g
Tween™ 60...................................................................................0.2g
MgSO 4 ·7H 2 O..............................................................................0.15g
KH 2 PO 4 .....................................................................................0.069g
Tween™ 80.................................................................................0.05g
EDTA..........................................................................................0.01g
CaCO 3 ........................................................................................4.0mg
Thiamine·HCl ............................................................................1.0mg
Vitamin B 12 .................................................................................1.0μg
pH 7.4–7.6 at 25°C
Preparation of Medium: Add components, except thiamine·HCl,
to distilled/deionized water and bring volume to 990.0mL. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Aseptically add 1.0mg of thiamine·HCl. Aseptically
distribute into sterile tubes or flasks.
Use: For the cultivation of Leptospira species.