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培养基配方
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Leptospira HiVeg Medium Base, Korthof, Modified with Rabbit Serum and Hemoglobin
[所属分类:培养基配方] [发布时间:2021-4-30] [发布人:李佳颐] [阅读次数:] [返回]
Leptospira HiVeg Medium Base, Korthof, Modified
with Rabbit Serum and Hemoglobin
Na 2 HPO 4 .....................................................................................0.88g
Plant peptone ................................................................................0.8g
KH 2 PO 4 .......................................................................................0.24g
CaCl 2 ...........................................................................................0.04g
KCl..............................................................................................0.04g
NaHCO 3 ......................................................................................0.02g
Rabbit serum, heat inactivated at 56°C ...................................80.0mL
Hemoglobin solution .................................................................8.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium, without rabbit serum, is available as a pre-
mixed powder from HiMedia.
Hemoglobin Solution:
Composition per 50.0mL:
Bovine hemoglobin.......................................................................1.0g
Preparation of Hemoglobin Solution: Add bovine hemoglobin to
distilled/deionized water and bring volume to 500.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium: Add components, except rabbit serum,
and hemoglobin solution, to distilled/deionized water and bring volume
to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into
tubes or flasks. Autoclave for 15 min at 10 psi pressure–115°C. Cool to
50°–56°C. Aseptically add 80.0mL of rabbit serum and 8.0mL of hemo-
globin solution. Mix thoroughly. Aseptically dispense into tubes.
Use : For the cultivation of Leptospira species.
with Rabbit Serum and Hemoglobin
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
NaCl..............................................................................................1.4gNa 2 HPO 4 .....................................................................................0.88g
Plant peptone ................................................................................0.8g
KH 2 PO 4 .......................................................................................0.24g
CaCl 2 ...........................................................................................0.04g
KCl..............................................................................................0.04g
NaHCO 3 ......................................................................................0.02g
Rabbit serum, heat inactivated at 56°C ...................................80.0mL
Hemoglobin solution .................................................................8.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium, without rabbit serum, is available as a pre-
mixed powder from HiMedia.
Hemoglobin Solution:
Composition per 50.0mL:
Bovine hemoglobin.......................................................................1.0g
Preparation of Hemoglobin Solution: Add bovine hemoglobin to
distilled/deionized water and bring volume to 500.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium: Add components, except rabbit serum,
and hemoglobin solution, to distilled/deionized water and bring volume
to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into
tubes or flasks. Autoclave for 15 min at 10 psi pressure–115°C. Cool to
50°–56°C. Aseptically add 80.0mL of rabbit serum and 8.0mL of hemo-
globin solution. Mix thoroughly. Aseptically dispense into tubes.
Use : For the cultivation of Leptospira species.
