Leptospira HiVeg Medium Base, Korthof, Modified with Rabbit Serum

[所属分类:培养基配方] [发布时间:2021-4-30] [发布人:李佳颐] [阅读次数:] [返回]

Leptospira HiVeg Medium Base, Korthof, Modified
with Rabbit Serum

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..............................................................................................1.4g
Na 2 HPO 4 .....................................................................................0.88g
Plant peptone.................................................................................0.8g
KH 2 PO 4 .......................................................................................0.24g
CaCl 2 ...........................................................................................0.04g
KCl..............................................................................................0.04g
NaHCO 3 ......................................................................................0.02g
Rabbit serum, heat inactivated at 56°C..................................100.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium, without rabbit serum, is available as a pre-
mixed powder from HiMedia.
Preparation of Medium: Add components, except rabbit serum, to
distilled/deionized water and bring volume to 900.0mL. Mix thoroughly.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 10 psi pressure–115°C. Cool to 50°–56°C. Aseptical-
ly add 100.0mL of rabbit serum. Mix thoroughly.
Use : For the cultivation of Leptospira species.