Lecithin Tween™ Medium (LT Medium)

[所属分类:培养基配方] [发布时间:2021-4-29] [发布人:李佳颐] [阅读次数:] [返回]

Lecithin Tween™ Medium
(LT Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Tween™ 80.................................................................................30.0g
Agar............................................................................................15.0g
Pancreatic digest of casein..........................................................10.0g
Peptic digest of animal tissue .....................................................10.0g
NaCl..............................................................................................5.0g
Lecithin.........................................................................................5.0g
Na 2 S 2 O 3 ·5H 2 O..............................................................................5.0g
Glycerol ........................................................................................3.0g
Histidine, free base .......................................................................1.0g
Glucose.........................................................................................1.0g
pH 7.5 ± 0.2 at 25°C
Antibiotic Solution:
Composition per 10.0mL:
5–Fluorocytosine ..........................................................................0.2g
Fosfomicin....................................................................................0.1g
Ticarcillin......................................................................................0.1g
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except antibiotic solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile anti-
biotic solution. Mix thoroughly. Pour into sterile Petri dishes in
20.0mL volumes.
Use: For the isolation and cultivation of multiresistant lipophilic
Corynebacterium species, especially Corynebacterium group JK found
primarily in infections in immunocompromised hosts and patients with
prosthetic valve endocarditis.