Lecithin Lipase Anaerobic Agar

[所属分类:培养基配方] [发布时间:2021-4-29] [发布人:李佳颐] [阅读次数:] [返回]

Lecithin Lipase Anaerobic Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................40.0g
Agar............................................................................................25.0g
Yeast extract..................................................................................5.0g
Na 2 HPO 4 ·12H 2 O...........................................................................5.0g
Glucose.........................................................................................2.0g
NaCl..............................................................................................2.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.1g
Egg yolk emulsion.................................................................100.0mL
pH 7.6 ± 0.2 at 25°C
Egg Yolk Emulsion:
Composition :
Chicken egg yolks............................................................................ 11
Whole chicken egg ............................................................................1
Preparation of Egg Yolk Emulsion:  Soak eggs with 1:100 dilu-
tion of saturated mercuric chloride solution for 1 min. Crack eggs and
separate yolks from whites. Mix egg yolks with 1 chicken egg. Filter
sterilize.
Preparation of Medium: Add components, except egg yolk emul-
sion, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile egg yolk
emulsion. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the isolation, cultivation, and differentiation of Clostridium
species based on lecithinase production and lipase production. Bacteria
that produce lecithinase appear as colonies surrounded by a zone of
insoluble precipitate. Bacteria that produce lipase appear as colonies
with a pearly iridescent sheen.