Lee’s Agar

[所属分类:培养基配方] [发布时间:2021-4-29] [发布人:李佳颐] [阅读次数:] [返回]

Lee’s Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................18.0g
Pancreatic digest of casein..........................................................10.0g
Yeast extract................................................................................10.0g
Lactose..........................................................................................5.0g
Sucrose..........................................................................................5.0g
CaCO 3 ...........................................................................................3.0g
K 2 HPO 4 .........................................................................................0.5g
Bromcresol Purple (0.2% solution) .........................................10.0mL
pH 7.0 ± 0.2 at 25°C
Bromcresol Purple Solution:
Composition per 10.0mL:
Bromcresol Purple ......................................................................0.02g
Preparation of Bromcresol Purple Solution:  Add Bromcresol
Purple to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except Bromcresol Pur-
ple solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Adjust pH to 7.0. Gently heat and bring to boiling. Au-
toclave for 20 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti-
cally add sterile Bromcresol Purple solution. Mix thoroughly. Pour into
sterile, chilled Petri dishes in 20–25mL volumes. Swirl flask while dis-
pensing to evenly suspend CaCO 3 . Dry plates at 30°C for 18–24 hr be-
fore use.
Use: For the isolation, cultivation, and enumeration of Lactobacillus
bulgaricus from yogurt.