Lecithin Lactose Agar

[所属分类:培养基配方] [发布时间:2021-4-29] [发布人:李佳颐] [阅读次数:] [返回]

Lecithin Lactose Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Pancreatic digest of casein..........................................................12.7g
Lactose........................................................................................10.0g
NaCl..............................................................................................5.5g
Peptic digest of animal tissue........................................................5.5g
Yeast extract..................................................................................3.9g
Pancreatic digest of heart muscle..................................................3.3g
Cornstarch.....................................................................................1.1g
Egg lecithin.................................................................................0.66g
L- Cysteine·HCl·H 2 O......................................................................0.5g
NaN 3 ..............................................................................................0.2g
Neomycin sulfate........................................................................0.15g
CaCl 2 ...........................................................................................0.05g
Bromcresol Purple ......................................................................0.02g
pH 6.8 ± 0.2 at 25°C
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Source:  This medium is available as a prepared medium from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes.

Use:  For the isolation, cultivation, and differentiation of histolytic
clostridia from clinical specimens based on lecithinase production and lac-
tose fermentation. It is especially useful for the differentiation of Clostrid-
ium perfringens, Clostridium sordelli, Clostridium novyi, Clostridium sep-
ticum, and Clostridium histolyticum. Bacteria that produce lecithinase
appear as colonies surrounded by an opalescent zone. Bacteria that ferment
lactose appear as colonies surrounded by a yellow zone.