LC Broth

[所属分类:培养基配方] [发布时间:2021-4-29] [发布人:李佳颐] [阅读次数:] [返回]

LC Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................10.0g
Pancreatic digest of casein..........................................................10.0g
Yeast extract..................................................................................5.0g
Glucose.........................................................................................1.0g
CaCl 2 ·2H 2 O (1M solution).........................................................5.0mL
MgSO 4 ·7H 2 O (1M solution)......................................................5.0mL
pH 7.4 ± 0.2 at 25°C
CaCl 2 ·2H 2 O Solution:
Composition per 100.0mL:
CaCl 2 ·2H 2 O ................................................................................14.7g
Preparation of CaCl 2 ·2H 2 O Solution:  Add CaCl 2 ·2H 2 O to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
MgSO 4 ·7H 2 O Solution:
Composition per 100.0mL:
MgSO 4 ·7H 2 O............................................................................24.65g
Preparation of MgSO 4 ·7H 2 O Solution:  Add MgSO 4 ·7H 2 O to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except CaCl 2 ·2H 2 O so-
lution and MgSO 4 ·7H 2 O solution, to distilled/deionized water and
bring volume to 990.0mL. Mix thoroughly. Adjust pH to 7.4. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically
add 5.0mL of sterile CaCl 2 ·2H 2 O solution and 5.0mL of sterile
MgSO 4 ·7H 2 O solution. Mix thoroughly. Aseptically distibute into ster-
ile tubes or flasks.
Use: For the cultivation of Escherichia coli.