LD Esculin HiVeg Agar (Lombard-Dowell Esculin Agar, HiVeg)

[所属分类:培养基配方] [发布时间:2021-4-29] [发布人:李佳颐] [阅读次数:] [返回]

LD Esculin HiVeg Agar
(Lombard-Dowell Esculin Agar, HiVeg)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................20.0g
Plant hydrolysate No. 1.................................................................5.0g
Yeast extract..................................................................................5.0g
NaCl..............................................................................................2.5g
Esculin ..........................................................................................1.0g
Ferric citrate..................................................................................0.5g
L -Cystine.......................................................................................0.4g
L -Tryptophan.................................................................................0.2g
Fe 4 (P 2 O 7 ) 3 ·H 2 O...........................................................................0.01g
Vitamin K 1 ..................................................................................0.01g
pH 7.5 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMe-
dia.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into
sterile Petri plates.
Use: For the cultivation of a wide variety of anaerobic bacteria. For the
differentiation of anaerobic bacteria based on esculin hydrolysis, H 2 S
production, and catalase production. Bacteria that hydrolyze esculin
appear as colonies surrounded by a red-brown to dark brown zone.
Bacteria that produce H 2 S appear as black colonies.