LBS™ Broth (Lactobacillus Selection Broth)

[所属分类:培养基配方] [发布时间:2021-4-29] [发布人:李佳颐] [阅读次数:] [返回]

LBS™ Broth
(Lactobacillus Selection Broth)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sodium acetate·3H 2 O..................................................................25.0g
Glucose.......................................................................................20.0g
Pancreatic digest of casein..........................................................10.0g
KH 2 PO 4 .........................................................................................6.0g
Yeast extract..................................................................................6.0g
Ammonium citrate........................................................................2.0g
Polysorbate 80 ..............................................................................1.0g
MgSO 4 ......................................................................................0.575g
FeSO 4 ........................................................................................0.034g
MnSO 4 ........................................................................................0.12g
Acetic acid, glacial...................................................................1.32mL
pH 5.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components, except acetic acid, to
distilled/deionized water and bring volume to 998.7mL. Mix thorough-
ly. Gently heat and bring to boiling. Add glacial acetic acid. Mix thor-
oughly. Gently heat while stirring and bring to 90°–100°C for 2–3 min.
Do not autoclave. Aseptically distribute into sterile tubes.
Use: For the selective isolation and cultivation of lactobacilli.