LB Modified Broth (ATCC Medium 1620)

[所属分类:培养基配方] [发布时间:2021-4-29] [发布人:李佳颐] [阅读次数:] [返回]

LB Modified Broth
(ATCC Medium 1620)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1030.4mL:

Tryptone......................................................................................10.0g
NaCl..............................................................................................5.8g
Yeast extract..................................................................................5.0g
NaCl Solution:
Composition per 100.0mL:
NaCl............................................................................................20.0g
Preparation of NaCl Solution:  Add NaCl to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
MgCl 2 Solution:
Composition per 10.0mL:
MgCl 2 ............................................................................................0.9g
Preparation of MgCl 2 Solution:  Add MgCl 2 to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for
15 min at 15 psi pressure–121°C.
CaCl 2 Solution:
Composition per 10.0mL:
CaCl 2 .............................................................................................0.5g
Preparation of CaCl 2 Solution:  Add CaCl 2 to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
Glucose Solution:
Composition per 100.0mL:
D -Glucose....................................................................................40.0g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components except salts and glucose
solutions to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Autoclave for 30 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add 16.8mL NaCl solution, 1.6 mL MgCl 2  so-
lution, 2.0mL CaCl 2  solution, and 10.0mL glucose solution. Mix thor-
oughly.
Use: For the cultivation of Escherichia coli (Migula) Castellani and
Chalmers.