LB Streptomycin Medium

[所属分类:培养基配方] [发布时间:2021-4-29] [发布人:李佳颐] [阅读次数:] [返回]

LB Streptomycin Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................10.0g
Pancreatic digest of casein..........................................................10.0g
Yeast extract..................................................................................5.0g
Streptomycin solution..............................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Streptomycin Solution:
Composition per 10.0mL:
Streptomycin.................................................................................0.2g
Preparation of Streptomycin Solution: Add streptomycin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except streptomycin
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Adjust pH to 7.0. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C. Aseptically add sterile streptomycin
solution. Mix thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use: For the cultivation of Escherichia coli.