LB Medium for Χ1776 with Tetracycline and Ampicillin

[所属分类:培养基配方] [发布时间:2021-4-29] [发布人:李佳颐] [阅读次数:] [返回]

LB Medium for Χ1776 with Tetracycline
and Ampicillin

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................10.0g
Pancreatic digest of casein..........................................................10.0g
Yeast extract..................................................................................5.0g
Antibiotic solution ...................................................................10.0mL
Glucose solution ......................................................................10.0mL
Diaminopimelic acid solution..................................................10.0mL
Thymidine solution..................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Antibiotic Solution:
Composition per 10.0mL:
Ampicillin...................................................................................0.01g
Tetracycline.................................................................................0.01g
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Glucose Solution:
Composition per 10.0mL:
D- Glucose......................................................................................0.8g
Preparation of Glucose Solution: Add  D- glucose to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Diaminopimelic Acid Solution:
Composition per 10.0mL:
DL- Diaminopimelic acid................................................................0.1g
Preparation of Diaminopimelic Acid Solution: Add diamin-
opimelic acid to distilled/deionized water and bring volume to 10.0mL.
Mix thoroughly. Filter sterilize.
Thymidine Solution:
Composition per 10.0mL:
Thymidine...................................................................................0.02g
Preparation of Thymidine Solution: Add thymidine to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components—except glucose solu-
tion, diaminopimelic acid solution, and thymidine solution—to dis-
tilled/deionized water and bring volume to 960.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C. Aseptically add sterile antibiotic solu-
tion, glucose solution, diaminopimelic acid solution, and thymidine
solution. Mix thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use:  For the cultivation and maintenance of Bacillus subtilis and
Escherichia coli.