Lauryl Tryptose Mannitol Broth with Tryptophan

[所属分类:培养基配方] [发布时间:2021-4-28] [发布人:李佳颐] [阅读次数:] [返回]

Lauryl Tryptose Mannitol Broth with Tryptophan

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................20.0g
Lactose..........................................................................................5.0g
NaCl..............................................................................................5.0g
K 2 HPO 4 .......................................................................................2.75g
KH 2 PO 4 .......................................................................................2.75g
Sodium lauryl sulfate....................................................................0.1g
L- Tryptophan.................................................................................0.2g
pH 6.8 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Distribute into tubes con-
taining an inverted Durham tube in 10.0mL volumes. Autoclave for 10
min at 10 psi pressure–115°C. Cool broth quickly to 25°C.
Use: For the detection of Escherichia coli in water samples.