LAVMm2 Medium

[所属分类:培养基配方] [发布时间:2021-4-28] [发布人:李佳颐] [阅读次数:] [返回]

LAVMm2 Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Lactalbumin hydrolysate.............................................................10.0g
Sodium acetate..............................................................................5.0g
MgCl 2 ·6H 2 O.............................................................................20.3mg
Nitrilotriacetic acid..................................................................19.1mg
CaCl 2 ........................................................................................11.1mg
FeSO 4 .....................................................................................0.152mg
Thiamine·HCl ..........................................................................0.05mg
Cupric acetate ..........................................................................0.04mg
Biotin .......................................................................................0.02mg

pH 8.0–8.1 at 25°C

Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5 with
Na 2 CO 3 . Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. The pH should be 8.0–8.1 after autoclaving.
Use: For the cultivation of Caryophanon latum.