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Lauryl Tryptose Broth with MUG (Lauryl Sulfate Broth with MUG) (LST-MUG) (BAM M77)
[所属分类:培养基配方] [发布时间:2021-4-28] [发布人:李佳颐] [阅读次数:] [返回]
Lauryl Tryptose Broth with MUG
(Lauryl Sulfate Broth with MUG)
(LST-MUG)
(BAM M77)
Lactose..........................................................................................5.0g
NaCl..............................................................................................5.0g
K 2 HPO 4 .......................................................................................2.75g
KH 2 PO 4 .......................................................................................2.75g
Sodium lauryl sulfate....................................................................0.1g
4-Methylumbelliferyl-β- D- glucuronide (MUG)..........................0.05g
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
containing an inverted Durham tube in 10.0mL volumes. Autoclave for
12 min at 15 psi pressure–121°C. Cool broth quickly to 25°C. For test-
ing water samples with 10.0mL volumes, prepare medium double
strength.
Use: For the detection of Escherichia coli in water and food samples
by a fluorogenic procedure.
(Lauryl Sulfate Broth with MUG)
(LST-MUG)
(BAM M77)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Pancreatic digest of casein..........................................................20.0gLactose..........................................................................................5.0g
NaCl..............................................................................................5.0g
K 2 HPO 4 .......................................................................................2.75g
KH 2 PO 4 .......................................................................................2.75g
Sodium lauryl sulfate....................................................................0.1g
4-Methylumbelliferyl-β- D- glucuronide (MUG)..........................0.05g
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
containing an inverted Durham tube in 10.0mL volumes. Autoclave for
12 min at 15 psi pressure–121°C. Cool broth quickly to 25°C. For test-
ing water samples with 10.0mL volumes, prepare medium double
strength.
Use: For the detection of Escherichia coli in water and food samples
by a fluorogenic procedure.
