Lactic Acid Bacteria Medium

[所属分类:培养基配方] [发布时间:2021-4-26] [发布人:李佳颐] [阅读次数:] [返回]

Lactic Acid Bacteria Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Glucose .......................................................................................10.0g
KH 2 PO 4 .........................................................................................5.0g
Peptone..........................................................................................5.0g
Sodium acetate·3H 2 O....................................................................5.0g
Yeast extract..................................................................................5.0g
Diammonium hydrogen citrate.....................................................2.0g
Tween™ 80...................................................................................1.0g
Sorbic acid....................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.5g
MnSO 4 ·4H 2 O................................................................................0.2g
FeSO 4 ·7H 2 O................................................................................0.05g
pH 5.3–5.4 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.3–5.4.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.
Use: For the isolation and cultivation of lactic acid bacteria from wine.