Lactic Acid Bacteria Broth

[所属分类:培养基配方] [发布时间:2021-4-26] [发布人:李佳颐] [阅读次数:] [返回]

Lactic Acid Bacteria Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sodium acetate............................................................................12.0g
Pancreatic digest of casein..........................................................10.0g
Yeast autolysate.............................................................................5.0g
Glucose .......................................................................................10.0g
Solution A..................................................................................5.0mL
Solution B..................................................................................5.0mL
pH 5.1 ± 0.2 at 25°C
Source:  Yeast autolysate is available from Oxoid Unipath.
Solution A:
Composition per 100.0mL:
K 2 HPO 4 .......................................................................................10.0g
KH 2 PO 4 .......................................................................................10.0g
Preparation of Solution A:  Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly.
Solution B:
Composition per 100.0mL:
MgSO 4 ·7H 2 O................................................................................4.0g
FeSO 4 ·7H 2 O..................................................................................0.2g
MnSO 4 ·H 2 O..................................................................................0.2g
NaCl..............................................................................................0.2g
Preparation of Solution B:  Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Lactobacillus malefermentans.