Lactic Acid Bacteria Selective Agar Base

[所属分类:培养基配方] [发布时间:2021-4-26] [发布人:李佳颐] [阅读次数:] [返回]

Lactic Acid Bacteria Selective Agar Base

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Casein enzymic hydrolysate.......................................................20.0g
Agar............................................................................................17.0g
Maltose .......................................................................................10.0g
Yeast extract..................................................................................5.0g
Fructose.........................................................................................5.0g
Glucose.........................................................................................5.0g
Potassium aspartate.......................................................................2.5g
Potassium glutamate.....................................................................2.5g
Betaine hydrochloride...................................................................2.0g
Diammonium hydrogen citrate.....................................................2.0g
MgSO 4 ·7H 2 O................................................................................2.0g
KH 2 PO 4 .........................................................................................2.0g
Liver concentrate..........................................................................1.0g
MnSO 4 ·2H 2 O..............................................................................0.66g
N-acetyl glucosamine....................................................................0.5g
Selective supplement solution ................................................20.0mL
pH 5.4 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Selective Supplement Solution:
Composition per 20.0mL:
2-Phenylethanol............................................................................3.0g
Cycloheximide.........................................................................10.0mg
Polysorbate 80 .........................................................................10.0mL
Preparation of Selective Supplement Solution:  Add compo-
nents to distilled/deionized water and bring volume to 20.0mL. Mix
thoroughly. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except selective sup-
plement solution, to distilled/deionized water and bring volume to
980.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add selective supplement solution.
Mix thoroughly. Pour into Petri dishes or aseptically distribute into
sterile tubes.
Use: For the selective isolation of lactic acid bacteria from beer and
brewing processes.