L15 Medium, Modified Leibovitz

[所属分类:培养基配方] [发布时间:2021-4-26] [发布人:李佳颐] [阅读次数:] [返回]

L15 Medium, Modified Leibovitz

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..............................................................................................8.0g
DL- Threonine.................................................................................0.6g
Sodium pyruvate...........................................................................0.6g
DL- Alanine ....................................................................................0.5g
L- Arginine, free base.....................................................................0.5g
KCl................................................................................................0.4g
L- Asparagine·H 2 O.........................................................................0.3g
L- Histidine, free base ....................................................................0.3g
L- Glutamine ..................................................................................0.3g
L- Isoleucine...................................................................................0.3g
L- Phenylalanine.............................................................................0.3g
L- Tyrosine .....................................................................................0.3g
DL- Methionine...............................................................................0.2g
DL- Valine.......................................................................................0.2g
Glycine..........................................................................................0.2g
L- Serine.........................................................................................0.2g
Na 2 HPO 4 , anhydrous....................................................................0.2g
CaCl 2 , anhydrous..........................................................................0.1g
L- Cysteine, free base.....................................................................0.1g
L- Leucine·HCl...............................................................................0.1g
MgCl 2 , anhydrous.....................................................................0.094g
D- Galactose.................................................................................0.09g
KH 2 PO 4 .......................................................................................0.06g
L- Tryptophan...............................................................................0.02g
Phenol Red..................................................................................0.01g
i-Inositol.....................................................................................2.0mg
Choline chloride.........................................................................1.0mg
D- Calcium pantothenate.............................................................1.0mg
Folic acid ...................................................................................1.0mg
Nicotinamide..............................................................................1.0mg
Pyridoxine·HCl..........................................................................1.0mg
Thiamine monophosphate·2H 2 O ...............................................1.0mg
Riboflavin-5-phosphate .............................................................0.1mg
pH 7.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Store
at 5°C.
Use: For the cultivation of oysters used for the growth of enterovi-
ruses.