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L. mono Confirmatory Agar Base (Listeria monocytogenes Confirmatory Agar, Base)
[所属分类:培养基配方] [发布时间:2021-4-26] [发布人:李佳颐] [阅读次数:] [返回]
L. mono Confirmatory Agar Base
(Listeria monocytogenes Confirmatory Agar, Base)
Agar............................................................................................12.0g
LiCl.............................................................................................10.0g
B.C. indicator............................................................................... 8.6g
Yeast extract..................................................................................6.0g
NaCl..............................................................................................5.0g
α-Methyl- D -mannoside ................................................................3.0g
Na 2 HPO 4 , anhydrous....................................................................2.5g
Listeria mono enrichment supplement II.................................10.0mL
Listeria mono selective supplement I......................................10.0mL
Listeria mono selective supplement II.....................................10.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium, without Listeria mono enrichment supplement
II, Listeria mono selective supplement I, and Listeria mono selective
supplement II, is available as a premixed powder from BioChemika.
Caution: LiCl is harmful. Avoid bodily contact and inhalation of va-
pors. On contact with skin wash with plenty of water immediately.
Listeria mono Enrichment Supplement II Solution:
Composition per 10.0mL:
L -phosphatidylinositol ..................................................................1.0g
Preparation of Listeria mono Enrichment Supplement II So-
lution: Add L -phosphatidylinositol to distilled/deionized water and
bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Listeria mono Selective Supplement I Solution:
Composition per 10.0mL:
Polymyxin B sulfate...............................................................76,700U
Preparation of Listeria mono Selective Supplement I Solu-
tion: Add polymyxin B sulfate to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Listeria mono Selective Supplement II Solution:
Composition per 10.0mL:
Ceftazidime .............................................................................20.0mg
Nalidixic acid, sodium salt ......................................................20.0mg
Amphotericin B .......................................................................10.0mg
Preparation of Listeria mono Selective Supplement II Solu-
tion: Add components to distilled/deionized water and bring volume
to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except Listeria mono
enrichment supplement II, Listeria mono selective supplement I, and
Listeria mono selective supplement II, to distilled/deionized water and
bring volume to 970.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 10.0mL Listeria mono enrichment supplement
II, 10.0mL Listeria mono selective supplement I, and 10.0mL Listeria
mono selective supplement II. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the selective and differential isolation of Listeria monocyto-
genes from clinical and food specimens.
(Listeria monocytogenes Confirmatory Agar, Base)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Special peptone...........................................................................30.0gAgar............................................................................................12.0g
LiCl.............................................................................................10.0g
B.C. indicator............................................................................... 8.6g
Yeast extract..................................................................................6.0g
NaCl..............................................................................................5.0g
α-Methyl- D -mannoside ................................................................3.0g
Na 2 HPO 4 , anhydrous....................................................................2.5g
Listeria mono enrichment supplement II.................................10.0mL
Listeria mono selective supplement I......................................10.0mL
Listeria mono selective supplement II.....................................10.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium, without Listeria mono enrichment supplement
II, Listeria mono selective supplement I, and Listeria mono selective
supplement II, is available as a premixed powder from BioChemika.
Caution: LiCl is harmful. Avoid bodily contact and inhalation of va-
pors. On contact with skin wash with plenty of water immediately.
Listeria mono Enrichment Supplement II Solution:
Composition per 10.0mL:
L -phosphatidylinositol ..................................................................1.0g
Preparation of Listeria mono Enrichment Supplement II So-
lution: Add L -phosphatidylinositol to distilled/deionized water and
bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Listeria mono Selective Supplement I Solution:
Composition per 10.0mL:
Polymyxin B sulfate...............................................................76,700U
Preparation of Listeria mono Selective Supplement I Solu-
tion: Add polymyxin B sulfate to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Listeria mono Selective Supplement II Solution:
Composition per 10.0mL:
Ceftazidime .............................................................................20.0mg
Nalidixic acid, sodium salt ......................................................20.0mg
Amphotericin B .......................................................................10.0mg
Preparation of Listeria mono Selective Supplement II Solu-
tion: Add components to distilled/deionized water and bring volume
to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except Listeria mono
enrichment supplement II, Listeria mono selective supplement I, and
Listeria mono selective supplement II, to distilled/deionized water and
bring volume to 970.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 10.0mL Listeria mono enrichment supplement
II, 10.0mL Listeria mono selective supplement I, and 10.0mL Listeria
mono selective supplement II. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the selective and differential isolation of Listeria monocyto-
genes from clinical and food specimens.
