L Medium with Tetracycline

[所属分类:培养基配方] [发布时间:2021-4-26] [发布人:李佳颐] [阅读次数:] [返回]

L Medium with Tetracycline

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................10.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................5.0g
Tetracycline solution................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Tetracycline Solution:
Composition per 10.0mL:
Tetracycline..............................................................................0.02mg
Preparation of Tetracycline Solution: Add tetracycline to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except tetracycline so-
lution, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Adjust pH to 7.0. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti-
cally add sterile tetracycline solution. Mix thoroughly. Aseptically
distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Escherichia coli.