Kreb’s Yeast Lactate Medium

[所属分类:培养基配方] [发布时间:2021-4-25] [发布人:李佳颐] [阅读次数:] [返回]

Kreb’s Yeast Lactate Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract................................................................................10.0g
Na 2 HPO 4 ·2H 2 O.............................................................................3.0g
KH 2 PO 4 .........................................................................................1.0g
Sodium lactate solution............................................................40.0mL
pH 7.0 ± 0.2 at 25°C
Sodium Lactate Solution:
Composition per 100.0mL:
Sodium lactate.............................................................................70.0g
Preparation of Sodium Lactate Solution: Add sodium lactate to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except sodium lactate
solution, to distilled/deionized water and bring volume to 960.0mL.
Mix thoroughly. Bring pH to 7.0. Autoclave for 15 min at 15 psi pres-
sure–121°C. Aseptically add 40.0mL of sterile sodium lactate solution.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Propionibacterium acidipropionici, Propi-
onibacterium freudenreichii, Propionibacterium intermedium, Propi-
onibacterium jensenii, Propionibacterium species, and Propionibacte-
rium thoenii.