KRANEP HiVeg Agar Base with Egg Yolk Emulsion

[所属分类:培养基配方] [发布时间:2021-4-25] [发布人:李佳颐] [阅读次数:] [返回]

KRANEP HiVeg Agar Base with Egg Yolk Emulsion

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Potassium thiocyanate ................................................................25.5g
Agar............................................................................................15.0g
Sodium pyruvate...........................................................................8.2g
LiCl...............................................................................................5.1g
Mannitol........................................................................................5.1g
Plant peptone ................................................................................5.0g
NaCl..............................................................................................5.0g
Plant extract..................................................................................1.5g
Yeast extract..................................................................................1.5g
NaN 3 ...........................................................................................0.05g
Cycloheximide..........................................................................0.041g
Egg yolk emulsion.................................................................100.0mL
pH 6.8 ± 0.2 at 25°C
Source: This medium, without egg yolk emulsion, is available as a
premixed powder from HiMedia.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Caution: Lithium Chloride is harmful. Avoid bodily contact and in-
halation of vapors. On contact with skin wash with plenty of water im-
mediately.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Egg Yolk Emulsion:
Composition per liter:
Egg yolks.................................................................................30.0mL
NaCl, 0.9% solution.................................................................70.0mL
Preparation of Egg Yolk Emulsion:  Soak eggs with 1:100 dilu-
tion of saturated mercuric chloride solution for 1 min. Crack 11 eggs
and separate yolks from whites. Mix egg yolks. Measure 30.0mL of
egg yolk emulsion and add to 70.0mL of 0.9% sterile NaCl solution.

Mix thoroughly. Warm to 45°–50°C.

Preparation of Medium: Add components, except egg yolk emul-
sion, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL of
egg yolk emulsion. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the isolation and enumeration of staphylococci from foods.