KRANEP Agar Base

[所属分类:培养基配方] [发布时间:2021-4-25] [发布人:李佳颐] [阅读次数:] [返回]

KRANEP Agar Base

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

KSCN..........................................................................................25.5g
Agar ............................................................................................18.3g
Sodium pyruvate...........................................................................8.2g
Pancreatic digest of gelatin...........................................................6.1g
LiCl...............................................................................................5.1g
Mannitol........................................................................................5.1g
Beef extract...................................................................................3.7g
NaN 3 ............................................................................................0.05g
Cycloheximide..........................................................................0.041g
Egg yolk emulsion.................................................................100.0mL
pH 6.8 ± 0.2 at 25°C
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Egg Yolk Emulsion:
Composition :
Chicken egg yolks............................................................................ 11
Whole chicken egg ............................................................................1
Preparation of Egg Yolk Emulsion:  Soak eggs with 1:100 dilu-
tion of saturated mercuric chloride solution for 1 min. Crack eggs and
separate yolks from whites. Mix egg yolks with 1 chicken egg.
Preparation of Medium: Add components, except egg yolk emul-
sion, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL of
egg yolk emulsion. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the isolation and enumeration of staphylococci from foods.